Cihan TAŞTAN1, Bulut YURTSEVER1 , Gözde SIR KARAKUŞ1, Derya DİLEK KANÇAĞI1, Sevda DEMİR2,Selen ABANUZ1, Utku SEYİS1, Mülazim YILDIRIM1, Recai KUZAY1
, Ömer ELİBOL3, Serap ARBAK4,Merve AÇIKEL ELMAS4, Selçuk BİRDOĞAN5, Osman Uğur SEZERMAN6, Ayşe Sesin KOCAGÖZ8,Koray YALÇIN1,, Ercüment OVALI1,
1 Acıbadem Labcell Cellular Therapy Laboratory, İstanbul, Turkey
2Genetic and Bioengineering Department, Faculty of Engineering, Yeditepe University, İstanbul, Turkey
3Acıbadem Altunizade Hospital, İstanbul, Turkey
4Department of Histology and Embryology, School of Medicine, Acıbadem Mehmet Ali Aydınlar University, İstanbul, Turkey
5Electron Microscopy Laboratory, Acıbadem Mehmet Ali Aydınlar University, İstanbul, Turkey
6Department of Biostatistics and Medical Informatics, School of Medicine, Acıbadem Mehmet Ali Aydınlar University,
İstanbul, Turkey
7Epigenetiks Genetics Bioinformatics Software Inc., İstanbul, Turkey
8Infectious Disease Unit, Acıbadem Altunizade Hospital, İstanbul, Turkey
9Pediatric Bone Marrow Transplantation Unit, Medical Park Göztepe Hospital, İstanbul, Turkey
Abstract: The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.
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